http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111662899-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 |
| filingDate | 2020-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111662899-B |
| titleOfInvention | A kind of linker peptide-mediated enzyme-immobilized BaPAD catalyst and its preparation method and application |
| abstract | The invention discloses an enzyme-immobilized BaPAD catalyst mediated by a connecting peptide, a preparation method and application thereof, and belongs to the field of catalyst preparation and application. In this method, the connecting peptide specifically adsorbed on zeolite is first inserted into the plasmid pET28a-bapad vector containing the gene encoding phenolic acid decarboxylase BaPAD through the Nco I/Xho I restriction site, and then pretreated by fusion enzyme The enzyme-immobilized BaPAD catalyst was immobilized on the Na-Y zeolite carrier, wherein the phenolic acid decarboxylase BaPAD was from Bacillus atrophaeus. The biocatalyst prepared by the present invention is used to catalyze FA to produce 4-VG. Compared with whole-cell catalytic FA to produce 4-VG, under the same conditions, the concentration and productivity of 4-VG are increased to 1.97M and 22.8g/L /h; and has good reusability, and can meet the industrial production requirements of 4‑VG. |
| priorityDate | 2020-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.