| abstract |
The invention belongs to the field of genetic engineering, and specifically relates to a psychrophilic, alkaline lipase PgLip1 and its encoding gene and enzymatic properties. The invention provides a psychrophilic, alkaline lipase PgLip1 derived from Pseudomonas guttabilis, the amino acid sequence of which is shown in SEQ ID NO.1, and the theoretical molecular weight is 27.4kDa. The psychrophilic and alkaline lipase of the present invention not only has the characteristics of psychrophilic and alkali resistance, but also has metal ion resistance and surfactant resistance, and has potential application value in the field of agricultural biotechnology. |