http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111662889-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P41-002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate | 2020-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111662889-B |
| titleOfInvention | Ketoreductase mutant for producing darunavir intermediate |
| abstract | The embodiment of the invention discloses a ketoreductase mutant for producing a darunavir intermediate, belongs to the technical field of biocatalysis methods and application, and particularly relates to a ketoreductase mutant for producing (2S,3S) -1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol, which is derived from wild ketoreductase of Starmerlla magnoliae, can convert 3S-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone into (2S,3S) -1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and has higher alcohol dehydrogenase activity compared with a wild sequence, has more than 90% similarity to SEQ ID No.8 and has one or more of the following characteristics: I54E, S85A and K182V, wherein the sequence of the ketoreductase mutant is SEQ ID NO. 2. The invention uses alcohols for coenzyme circulation, the concentration of the substrate is as high as 110g/L, the dosage of the substrate/NADP dosage is as high as 1100:1, the circulating times of the coenzyme are high, and the downstream application range is effectively enlarged. |
| priorityDate | 2020-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 569.