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filingDate 2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111650383-A
titleOfInvention Chemiluminescence immunoassay based on fluorescent dye as internal standard and its application
abstract The embodiment of the present invention discloses a chemiluminescence immunoassay based on a fluorescent dye as an internal standard and an application thereof. The invention relates to the research and application of novel nanomaterials and the fields of preventive and diagnostic medical testing, and solves the problem that the precision of the existing chemiluminescence immunoassay method needs to be improved. In the present invention, an antibody of an antigen to be tested is coated on the carboxyl group of a fluorescent magnetic microsphere to form an antibody-fluorescent magnetic microsphere, another antibody of an antigen to be tested is labeled with a luminescent substance, and the antibody-fluorescent magnetic microsphere is added to the antibody-fluorescent magnetic microsphere. The antigen to be tested forms a luminescent antibody-antigen-to-be-tested-fluorescent magnetic microsphere immune complex. After cleaning and separation, the signal of the complex is read to obtain the fluorescence value, and the excitation solution is automatically pumped into the complex to generate a chemiluminescence signal, and the chemiluminescence is measured. The luminescence intensity of the signal is calibrated, and the concentration of the antigen to be tested is calculated according to the calibrated luminescence intensity. The invention can reduce random errors generated by the system and improve the precision of analysis.
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