Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a157333b195d3f1e6081571ea7df5c97 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db9efde660b78f98b102046e09d07ab7 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4712 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6439 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6887 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-587 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
filingDate |
2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adf75e111b94e90c296f92f53ad54112 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d0a667f9651250ecc352ca86a660370 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de5c0ef729ae181110288f5dcba1cfc2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fcd73427bdf1e9c9ba19a82f0caa525f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c67428da05e9fef3370d2db6956b970 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b1892e9586dbf12ac159e2f00112ce8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82d4e32e553911e3e3f363ab921c9657 |
publicationDate |
2020-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-111650383-A |
titleOfInvention |
Chemiluminescence immunoassay based on fluorescent dye as internal standard and its application |
abstract |
The embodiment of the present invention discloses a chemiluminescence immunoassay based on a fluorescent dye as an internal standard and an application thereof. The invention relates to the research and application of novel nanomaterials and the fields of preventive and diagnostic medical testing, and solves the problem that the precision of the existing chemiluminescence immunoassay method needs to be improved. In the present invention, an antibody of an antigen to be tested is coated on the carboxyl group of a fluorescent magnetic microsphere to form an antibody-fluorescent magnetic microsphere, another antibody of an antigen to be tested is labeled with a luminescent substance, and the antibody-fluorescent magnetic microsphere is added to the antibody-fluorescent magnetic microsphere. The antigen to be tested forms a luminescent antibody-antigen-to-be-tested-fluorescent magnetic microsphere immune complex. After cleaning and separation, the signal of the complex is read to obtain the fluorescence value, and the excitation solution is automatically pumped into the complex to generate a chemiluminescence signal, and the chemiluminescence is measured. The luminescence intensity of the signal is calibrated, and the concentration of the antigen to be tested is calculated according to the calibrated luminescence intensity. The invention can reduce random errors generated by the system and improve the precision of analysis. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115524485-A |
priorityDate |
2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |