| abstract |
The invention relates to a method for detecting the activity of wheat grain polyphenol oxidase and its application, and belongs to the technical field of wheat breeding. The detection method of the present invention includes the following steps: 1) mixing the flour with the reaction reagent, shaking for 30 minutes, to obtain a reaction solution; 2) leaving the reaction solution obtained in step 1) at 0° C. for 5 minutes to terminate the reaction, and obtaining a termination Reaction solution; 3) Centrifuge the termination reaction solution obtained in step 2), take the supernatant and add it to the enzyme labeling plate, use the reaction reagent as a control, measure the absorbance value at 475 nm with a microplate reader, and calculate the polyphenol oxidase activity value. The detection method of the invention is accurate, fast, simple, has good repeatability and high stability, can realize the detection of PPO activity in wheat grains with a wide variation range and rich genetic basis, and screen out new wheat varieties with low PPO activity that exist stably in different environments. Variety. |