| abstract |
The invention discloses a construction method and application of a Ces2 knockout rat model for drug metabolism research. The method for constructing the Ces2 knockout rat model includes the steps of selection of gene knockout targets, sgRNA synthesis, embryo microinjection, rat feeding and breeding, etc., to obtain a homozygous Ces2 knockout rat model. Then, the homozygous Ces2 knockout rat model was tested for Ces2 expression from the mRNA level, and the metabolic function was verified to prove that the Ces2 knockout rat model was successfully constructed. The present invention utilizes the above method to successfully construct Ces2a/j, Ces2c and Ces2a/c/j gene knockout rat models, which can not only be used as an important tool for Ces2-related drug metabolism research, but also an important animal model for studying Ces2's physiological function. |