| abstract |
The invention belongs to the field of molecular biology and discloses an optimized PCV2d ORF2 gene and a preparation method for virus-like particles thereof. In the present invention, after the coding gene sequence encoding the porcine circovirus 2d subtype Cap protein is optimized, an expression engineering bacteria expression system is constructed to express the Cap protein. Under suitable expression conditions, the expression strain can express a large amount of soluble and active porcine circovirus 2d subtype Cap protein, and the expressed protein is purified by a monoclonal antibody column to obtain a high-purity and high-concentration Cap protein, and This protein can form virus-like particles. The invention realizes the efficient soluble expression of the full-length Cap protein of porcine circovirus 2d subtype in the prokaryotic expression system, the operation is simple, the cost is low, the prepared monoclonal antibody column can be used repeatedly, and the purified Cap protein has high concentration And high purity, and can spontaneously form virus-like particles, good immunogenicity, suitable for industrial applications. |