| abstract |
The invention provides a crRNA and a kit for detecting African swine fever virus, belonging to the technical field of virus detection. The crRNA described in the present invention is the crRNA that guides CRISPR-Cas12a to specifically bind to the African swine fever target DNA; the kit of the present invention includes the target DNA, crRNA, CRISPR-Cas12a, optimized detection buffer, ssDNA Probe and RNase inhibitor; after CRISPR-Cas12a recognizes the target molecule African swine fever DNA under the guidance of crRNA, CRISPR-Cas12a, crRNA and target DNA molecule combine into a complex to cut the ssDNA fluorescent probe in the detection system, producing a large number of Fluorescence can be detected. The kit of the invention has good detection specificity, high sensitivity, simple operation and short time consumption. |