| abstract |
The invention relates to the field of genetic engineering, and in particular, provides a gRNA that specifically recognizes the porcine COL1A1 gene and biological materials, kits and applications thereof. The nucleotide sequence responsible for identifying the target fragment region in the gRNA that specifically recognizes the pig COL1A1 gene is the sequence shown in SEQ ID NO.1, and the gRNA provided by the present invention can identify the downstream of the pig COL1A1 gene coding region as shown in SEQ ID NO. target sequence. Through the mediation of the gRNA, Cas9 can target the end of the 51st exon of the COL1A1 gene on the pig genome to generate a double-strand break, and the cutting efficiency can reach 22%, thereby realizing the editing of the COL1A1 gene, such as amino acid modification , replace, or insert an exogenous gene expression cassette at this site to achieve stable and efficient expression of the exogenous gene. |