http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111647583-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-50 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 |
| filingDate | 2020-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111647583-B |
| titleOfInvention | Preparation method of neutral protease |
| abstract | The invention belongs to the technical field of fermentation, and provides a preparation method of neutral protease, which comprises the following steps: A. inoculating the bacillus subtilis seed solution into a fermentation culture medium for fermentation culture at the temperature of 25-28 ℃ for 14-16 h to obtain a primary fermentation liquid; B. adding a supplementary culture medium into the primary fermentation liquid for culturing for 12-15 h to obtain a fermentation liquid; C. flocculating, fine filtering, ultrafiltering, concentrating and stabilizing the fermentation liquor to obtain a neutral protease finished product; the pH value of the fermentation medium is 6.5-6.8, and the fermentation medium comprises the following components in parts by weight: 30-50 parts of sweet potato starch, 20-30 parts of bean cake powder, 5-10 parts of pumpkin powder, 1.2-1.5 parts of magnesium humate, 0.8-1.2 parts of calcium caseinate, 0.5-1.2 parts of dipotassium hydrogen phosphate, 1-1.5 parts of ammonia chloride, 1-1.3 parts of sodium nitrate and 400-500 parts of water. By adopting the technical scheme, the problem that the enzyme activity of the neutral protease produced by a microbial fermentation method in the prior art is low is solved. |
| priorityDate | 2020-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.