| abstract |
The method for separating and purifying N-acetylglucosamine of the invention belongs to the technical field of biological engineering. In the present invention, the raw material liquid containing N-acetylglucosamine obtained by microbial fermentation or hydrolysis of chitin is subjected to flocculation pretreatment, and suspended solids such as microorganisms, proteins and polysaccharides are removed by continuous centrifugation or pressure filtration to obtain clarification. liquid. Then, impurities such as charged organic molecules and inorganic salts are removed by double-stage ion exchange chromatography. Efficient removal of water through membrane concentration to increase the concentration of the target product. Finally, concentrated and crystallized by spray drying or further evaporation, and finally dried to obtain N-acetylglucosamine crystals with a purity of more than 99%. |