| abstract |
The invention provides a kit and a detection method for detecting ALK gene mutation in peripheral blood circulating tumor cells of non-small cell lung cancer patients, belonging to the technical field of molecular biology. The kit provided by the present invention includes diluent, 1 mL of decolorization solution, staining solution A, staining solution B, ALK-D5F3 (rabbit monoclonal antibody), goat anti-rabbit IgG/HRP, 0.1% Triton X-100, 0.3% H 2 O 2 100, Reagent A, Reagent B, 6×PBS buffer. The detection method provided by the present invention can detect the expression of ALK in patients with advanced or recurrent non-small cell lung cancer without obtaining tissue samples by needle biopsy. And can detect in real time. The method provided by the invention can avoid false positive results caused by edge effects that may occur in the dyeing process, has good stability, reduces cell loss and improves detection accuracy. |