| abstract |
The present invention provides a method for detecting CEA gene mutation in peripheral blood circulating tumor cells of colorectal cancer patients. Carcinoembryonic antigen, goat anti-mouse IgG/HRP 100 μL, 0.1% Triton X‑100 100 μL, 0.3% H 2 O 2 100 μL, Reagent A, Reagent B, 6×PBS buffer 60 mL). By collecting peripheral blood, pre-processing peripheral blood, enriching CTCs in peripheral blood, staining, and finally using immunohistochemistry to detect gene expression, the cancer stage and recurrence of patients can be determined. |