| abstract |
The invention provides a primer, a sex identification method and a kit for identifying the sex of cassowaries, and relates to the technical field of biological detection and artificial breeding. The sequences of primers for identifying Cassowaries of the present invention are shown in SEQ ID No.1 and SEQ ID No.2. The gender identification method of the present invention comprises the following steps: S1, extracting the DNA of blood or feathers of cassowaries; S2, taking the DNA extracted in step S1 as a template, using the above-mentioned primers RASEF-F1 and RASEF-R1 to carry out PCR Amplify, and perform agarose detection on the PCR product; S3. Sequence the PCR amplified product, and determine the sex of Crane according to the linked single nucleotide difference of the obtained sequence. The primers and the identification method of the invention can efficiently, quickly and accurately identify the sex of cassowaries. |