http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111621518-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
filingDate | 2020-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111621518-B |
titleOfInvention | Construction method and application of agrobacterium rhizogenes-mediated transgenic poplar |
abstract | The invention discloses a construction method and application of agrobacterium rhizogenes-mediated transgenic poplar, and relates to the technical field of transgenosis. Specifically, the construction method comprises the steps of inoculating the explant on a solid basic culture medium in which agrobacterium rhizogenes colonies are pre-cultured, screening and culturing the explant after co-culture until the explant is impregnated with the agrobacterium rhizogenes, wherein the construction method is short in time consumption, liquid shaking is not needed, the OD value of a bacterial liquid is not needed to be controlled, the average operation time of each plant is not more than 5min in the impregnating process, the experiment time and the operation difficulty are obviously reduced, the transformation efficiency is high, and a novel method is provided for cultivation of transgenic plants. |
priorityDate | 2020-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.