http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111606994-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4bb019429bd39519ffe85008c129bb29 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-06 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 |
| filingDate | 2020-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9ec34ce9e9058d1e3af5af797f86528 |
| publicationDate | 2020-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111606994-A |
| titleOfInvention | Preparation method of non-fusion type anti-desmoglein DSG3 rabbit monoclonal antibody |
| abstract | The invention discloses a preparation method of a non-fusion type anti-desmoglycoprotein DSG3 rabbit-derived monoclonal antibody, comprising the following steps: 1) selecting a suitable immunized animal and immunizing it with DSG3; 2) extracting the blood of the successfully immunized animal , collect peripheral blood mononuclear cells, and isolate the B lymphocyte population that can secrete DSG3 antibody; 3) Build a yeast display library of rabbit-derived single-chain antibody; 4) Build a DSG3 rabbit-derived monoclonal antibody expression vector library; 5) Build a DSG3 rabbit Source monoclonal antibody yeast expression library: transform the Pichia pastoris expression vector PZ recombinant plasmid into yeast expression strain X33, and screen the X33 recombinant transformed monoclonal bacteria that can secrete DSG3 antibody; 6) Obtain X33 recombinant transformation that can stably secrete DSG3 antibody Monoclonal strain; 7) Purify the obtained DSG3 rabbit monoclonal antibody. The preparation method of the invention can prepare stable DSG3 antibody, has industrial reproducibility, and is suitable for industrialization promotion and application. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115261401-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115261401-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116496395-A |
| priorityDate | 2020-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 366.