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publicationDate 2020-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111595995-A
titleOfInvention Simultaneous determination of 12 antioxidants and free sulfur by ultra-high performance liquid chromatography
abstract The invention discloses a method for simultaneously detecting 12 kinds of antioxidants and free sulfur by using ultra-high performance liquid chromatography. The standard solution and the sample solution are respectively subjected to gradient elution by the ultra-high performance liquid chromatography method to obtain the standard solution and the sample solution. chromatogram, and then compare the retention time of each chromatographic peak in the chromatogram of the sample solution with the chromatographic peak of the standard solution, and qualitatively analyze the free sulfur and 12 antioxidants in the sample solution, and calculate the peak area of each chromatographic peak. The content of free sulfur and 12 antioxidants in the sample to be tested is obtained; the detection conditions of ultra-high performance liquid chromatography are: the chromatographic column is Shim‑pack GIST‑HP C18, the size is 150 mm×2.1 mm×3 μm; the mobile phase A is methanol, mobile phase B is 0.1% phosphoric acid aqueous solution; column temperature is 35-45 °C, injection volume is 5-7 μL, and detection wavelength is 230 nm. The detection method can realize the effective separation and simultaneous detection of free sulfur and 12 kinds of antioxidants within 26 minutes, and has good detection accuracy, less solvent consumption and low analysis and detection cost.
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