http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111593053-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70521 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-26 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57492 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1048 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574 |
| filingDate | 2020-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111593053-B |
| titleOfInvention | DNA aptamer for identifying human programmed death receptor 1, method and application |
| abstract | The invention discloses a DNA aptamer for identifying a human programmed death receptor 1, a method and application, and belongs to the field of biochemical detection. Screening aptamers by using a Cell-SELEX technology, transfecting L02 cells with the constructed pDisplay/PD-1N surface display expression plasmid, and carrying out resistance screening by using G418 to obtain a Cell line L02-PD-1N for stably expressing PD-1N. And screening by taking the L02-PD-1N cell as a target, performing repeated multiple rounds of incubation-elution-amplification by using the oligonucleotide library and the cell line, performing reverse screening by using the cell which does not express PD-1N, and finally obtaining the ssDNA (single stranded deoxyribonucleic acid), namely the aptamer, capable of binding the PD-1N with high affinity by identifying the enriched ssDNA sub-library, sequencing and comparing affinity. The invention provides a DNA aptamer for identifying human PD-1N, which takes the aptamer as a core, can utilize biotin, fluorescent markers and the like to identify PD-1 molecules on the surfaces of T lymphocytes in cells or tissues, and has potential application prospects in diagnosis and treatment of tuberculosis, tumors and the like. |
| priorityDate | 2020-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.