http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111579702-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c9582d4716c9a3de701f9d90781e99df |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8831 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8818 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
| filingDate | 2019-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6092aa8ca2e3c490ea2122702e15e0d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c43807237d2383ab68f2f7819187790 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ebda894b3b4a80f6f455519774c3741 |
| publicationDate | 2020-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111579702-A |
| titleOfInvention | A kind of detection method of protein amino acid sequence coverage |
| abstract | The present invention provides a method for detecting the coverage of protein amino acid sequence. The detection method of the protein amino acid sequence coverage includes protein reductive alkylation, proteolysis, polypeptide desalting, liquid mass detection and data analysis, wherein, the proteolysis is to obtain a peptide segment by adding elastase for single digestion, and the elastase and the pending The mass ratio of the detected proteins was 1:(20~100). The detection method of the protein amino acid sequence coverage of the present invention adopts elastase to realize single enzyme cleavage, and does not need to simulate the prediction of enzyme cleavage to select the corresponding enzyme; does not need multiple enzyme cleavage, only one enzyme; The hydrophilic polypeptide will be missed, and the coverage will be improved; the detection method of the present invention has the advantages of quickness and simplicity, less workload, short detection period and economical price, and can reach 100% amino acid sequence coverage. |
| priorityDate | 2019-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 185.