| abstract |
The invention discloses an LAMP detection kit for actinobacillus pleuropneumoniae, which is provided with a detection primer group and an internal standard primer group; the detection primer group comprises a detection outer primer pair, a detection inner primer pair and a detection ring primer pair, and the internal standard primer group comprises an internal standard outer primer pair, an internal standard inner primer pair and an internal standard ring primer pair. The method has the advantages of high speed, high efficiency, simple and convenient operation, high specificity, high sensitivity, low cost, no need of expensive instruments, suitability for field detection and the like, and more importantly, the method improves the detection accuracy, timely discovers the false negative result in the detection and can effectively prevent various accidents caused by the false negative detection result. |