http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111574599-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1609f7e3e68d99f9d929df60d52f4bf |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96411 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-325 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-325 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 |
| filingDate | 2020-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d86d8e95ee74e23c9e671225c2267210 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0c281c176bb95daed5b7539610e8913 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58638bb27c4537cb99651a30a8cf6af6 |
| publicationDate | 2020-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111574599-A |
| titleOfInvention | A toxin modification method to solve the excessive enzymatic hydrolysis of insecticidal toxins by insect intestinal digestive enzymes |
| abstract | The invention provides a toxin transformation method for solving the problem of over-enzymatic hydrolysis of insecticidal toxins by insect intestinal digestive enzymes. It uses PeptideCutter or fluorescent peptide enzyme activity assay or multiplex substrate mass spectrometry (MSP‑MS) to determine the specific cleavage site of insect intestinal digestive enzymes; The transformation site of the non-functional region; finally, the selected multiple sites are simultaneously mutated into amino acids that are not recognized by intestinal proteases. The transformation method of the invention can simultaneously perform accurate mutation on multiple protease cleavage sites on the toxin, significantly improve the insecticidal activity to the target insect, and has the advantages of strong specificity, simple operation and the like. This provides a favorable way for the biological control of agricultural and forestry pests and the management of their resistance. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112225786-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111647058-A |
| priorityDate | 2020-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.