| abstract |
The invention relates to the field of biology, and specifically discloses a preparation method for constructing a biofilm of tissue-engineered retinal pigment epithelium, comprising the following steps: S1: uniformly dissolving polycaprolactone, gelatin and graphene oxide in polar In the solvent, a mixed solution is obtained, and the mass percentage concentrations of polycaprolactone, gelatin and graphene oxide in the mixed solution are respectively 69.6%, 29.8% and 0.6%; S2: The mixed solution obtained by S1 is placed in a high-voltage generator connected to Electrospinning was performed in the micro-injection pump to obtain biofilms. The invention can prepare a nanofiber composite membrane that not only supports the growth and function of ESC-RPE cells, but also takes into account biocompatibility and operative operability. |