| abstract |
The invention discloses a DNA methylation kit for colorectal cancer detection, a method of use and application thereof. The kit includes specific primers and probes for detecting or measuring the methylation status or level of one or more specific genes, SEPT9, NDRG4 and ACTB, in the DNA of the test sample. The kit of the invention can significantly improve the positive detection rate and specificity (true negative rate) of early-stage colorectal cancer, and can increase the detection sensitivity of the biomarker to picogram/nanogram DNA molecules. |