http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111560384-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N63-60 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01P3-00 |
filingDate | 2020-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111560384-B |
titleOfInvention | Application of gene FoRnt in regulation and control of pathogenicity of banana fusarium wilt |
abstract | The invention discloses application of a gene FoRnt in regulation and control of pathogenicity of banana vascular wilt pathogens, and belongs to the field of plant genetic engineering. The invention constructs a gene knockout vector and introduces the gene knockout vector into a protoplast of the fusarium oxysporum f.sp.cubense; knocking out the gene from fusarium oxysporum f.sp.cubense by using a homologous recombination method to obtain a knock-out mutant delta FoRnt; a gene complementation vector is constructed and is introduced into the delta FoRnt protoplast to obtain a complementation mutant delta FoRnt-com. Experiments prove that compared with wild type, the delta FoRnt has no significant difference in spore morphology, hypha morphology, high penetration resistance, oxidative stress resistance and the like; however, the deletion of the FoRnt causes a significant reduction in the pathogenicity of the FoRnt; the invention proves that FoRnt is necessary for pathogenicity of banana fusarium wilt. The research of the people is helpful to deeply elucidate the pathogenic molecular mechanism of the banana fusarium oxysporum and provides a target gene for developing an effective bactericide. |
priorityDate | 2020-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 232.