http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111549104-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682 |
filingDate | 2020-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111549104-B |
titleOfInvention | A non-diagnostic method for the detection of circRNAs based on DNA nanoribbons of long-chain DNA scaffolds |
abstract | The invention discloses a non-diagnostic detection method for circRNA based on DNA nanobelts of long-chain DNA scaffolds, comprising the following steps: ( 1 ) designing a circular DNA template containing hairpin probes H1 and There are two installation sites for the hairpin probe H 2 , and the sequence fragment that can specifically recognize the target BSJ site; (2) the circular DNA is used as a template; (3) the hairpin probe H 1 and the hairpin probe The needle H2 binds to long-chain DNA through self-assembly, and the hairpin probe H2 contains a fluorophore HEX and a quenching group BHQ1. (4) Finally, a BSJ site-driven long-chain-based DNA nanoribbons of DNA scaffolds. The DNA nanobelt of the present invention can specifically identify the target circRNA, and can be used for the direct analysis of circRNA in complex biological samples. |
priorityDate | 2020-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.