http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111549050-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-02 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 |
filingDate | 2020-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111549050-B |
titleOfInvention | Vitreoscilla hemoglobin expression frame suitable for bacillus and application |
abstract | The invention belongs to the field of biotechnology and fermentation engineering, and provides a vitreoscilla hemoglobin expression frame suitable for bacillus and application thereof, wherein vitreoscilla hemoglobin VHb is connected with a signal peptide SPywbN of YwbN in bacillus subtilis, a bacillus subtilis promoter P43 and an amylase terminator TamyL in bacillus licheniformis to form a VHb protein expression frame P43-SPywbN- vhb‑ TamyL. The expression cassette is applied to three kinds of bacillus (bacillus subtilis 168, bacillus amyloliquefaciens LX-12 and bacillus licheniformis DW2) to respectively express poly-gamma-glutamic acid and illite in comparison with the conventional VHb strain which is singly expressed in an enhanced mannerThe yield of the subtilin A and the bacitracin is improved by 21.85%, 18.77% and 23.40%, and the invention provides theoretical guidance for the efficient expression and wide application of the vitreoscilla hemoglobin VHb in bacillus. |
priorityDate | 2020-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.