http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111521705-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-562 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-062 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-324 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-30 |
| filingDate | 2020-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111521705-B |
| titleOfInvention | Method for enriching bile acid in serum |
| abstract | The invention relates to a method for enriching bile acid in serum, which comprises the following steps: the serum sample is pretreated to a certain degree, then passes through a Solid Phase Extraction (SPE) small column filled with a specific enrichment purification material, and the enrichment purification sample elution solution is obtained by optimizing the proportion and the dosage of a solvent used in the preparation process, and is subjected to high performance liquid chromatography-tandem mass spectrometry detection and analysis. The specific purifying material is prepared by bonding polar modified phenyl filler with silica gel as a matrix, and by utilizing the hydrophobic effect of a phenyl bonding phase and the steroid multi-ring plane of bile acid and the n-pi effect, the polar group and the polar part of the bile acid generate hydrogen bond and electrostatic effect, so that the specific enrichment and purification of various bile acids in serum are realized. The method has the advantages of simple operation process, clean sample treatment, high detection sensitivity of the high performance liquid chromatography-tandem mass spectrometry, good selectivity, accurate quantification and wide application value. |
| priorityDate | 2020-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.