http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111518790-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 |
| filingDate | 2018-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111518790-B |
| titleOfInvention | Sucrose hydrolase mutant and preparation method and application thereof |
| abstract | The invention discloses sucrose hydrolase, and belongs to the field of genetic engineering and enzyme engineering. The invention modifies sucrose hydrolase derived from Xanthomonas axopodis v. glycerides, Janthinobacterium agaricidinium NBRC 102515 and Caulobacter crescentus NA1000 CB15, and carries out site-directed mutagenesis on serine residues at 271 th position, 279 th position or 281 th position respectively, and the obtained single mutant enzyme has higher transglycosylation activity than wild sucrose hydrolase. The invention is helpful for the research on glycoside hydrolase transglycosylation and hydrolysis mechanism, and can also be applied to the industrial production of polysaccharide by glycoside hydrolase. |
| priorityDate | 2018-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 495.