http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111518742-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 |
| filingDate | 2020-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111518742-B |
| titleOfInvention | Nano-scale single exosome separation method |
| abstract | The invention discloses a method for separating nano-scale single exosomes, which fully dilutes cell culture solution containing exosomes; adding superparamagnetic amino magnetic bead diluent, oscillating and mixing at low frequency, and coupling the nano magnetic beads with the exosomes; transferring the coupling solution to the surface layer of the nanopore array microchip; placing the microchip on a shaking table, and carrying out low-speed and small-angle oscillation to uniformly distribute the coupling solution on the surface layer of the chip; placing a magnetic source on the lower surface of the microchip, and enabling a magnetic bead exosome couplet to enter the nanopore array in a progressive magnetic adsorption mode; and under the action of larger magnetic force, keeping stable adsorption of the magnetic bead exosome couplet, washing the upper surface of the substrate by using buffer solution, standing to obtain the single exosome-containing nano microchip, and directly using the single exosome-containing nano microchip for subsequent detection. The invention is based on the nano-pore micro-array microchip with high-precision screening function, and can realize the acquisition of high-purity single exosome by magnetic bead coupling exosome and gradual magnetic adsorption. |
| priorityDate | 2020-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.