http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111514869-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-285 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3804 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-285 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-38 |
| filingDate | 2020-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111514869-B |
| titleOfInvention | Preparation method of affinity chromatography medium for separating and purifying alpha-glucosidase and separation and purification method thereof |
| abstract | The invention provides a preparation method of an affinity chromatography medium for separating and purifying alpha-glucosidase and a method for separating and purifying the alpha-glucosidase, wherein the preparation method of the affinity chromatography medium comprises the following steps: s1, firstly, adopting absorbent cotton as a substrate, and preparing aldehyde-based absorbent cotton through an aldehyde reaction of a sodium periodate solution; s2, coupling acarbose serving as an affinity ligand to the aldehyde absorbent cotton prepared in the step S1 to prepare a coupling product; s3, reducing the coupling product obtained in the step S2 by adopting a sodium borohydride solution again to prepare a stable affinity chromatography medium; s4, washing the affinity chromatography medium prepared in the step S3 with distilled water, draining, and storing in ethanol solution for later use. The preparation method provided by the invention has the advantages of easily obtained raw materials, low price, simple operation, stable and reliable purification method, great application prospect and good economic and social benefits. |
| priorityDate | 2020-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.