http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111505179-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-89 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-74 |
filingDate | 2020-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111505179-B |
titleOfInvention | Method for detecting biopterin in marine water body |
abstract | The invention provides a method for detecting biopterin in a marine water body, which comprises the following steps: pretreatment: filtering a water sample, then discarding the filtrate, taking particles, adding cell lysate into the particles and crushing the particles to obtain a lysate; adjusting the pH value of the dissolution liquid, and extracting to obtain a water phase containing biopterin; 2) detecting the aqueous phase containing biopterin by reverse phase high performance liquid chromatography; wherein, the reversed-phase high performance liquid chromatography adopts isocratic elution, and the mobile phase is methanol and water. The method for detecting the content of biopterin in the marine water body provided by the embodiment of the invention effectively improves the detection sensitivity through the pretreatment under specific conditions, further reduces the detection limit, can adapt to the current situation of low content of the marine water body, has high accuracy, and has important significance for disclosing the regulation and control process of biopterin in marine plankton, particularly the life activity of micro organisms and enhancing the understanding of marine chemistry. |
priorityDate | 2020-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 586.