Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a10a6e256f4419ad73a8993eed237325 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2560-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-355 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5695 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-4077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-24 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-24 |
filingDate |
2013-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5baaf31a3fbc69f5b9af33de63211c5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_358808c90bc589dcc7ac87c7114e68c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef6c8710875e1833dcf236b633871b5c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f225e0da07f74faee9bca1e3c1e7b263 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_059981fe3f8e177d01b94506a7adf513 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_593fe63061dbc2911ae9c78805e5278b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd4e3962e8ef332fcbbfe5134ec8e360 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a721d7784482917735a939aece873448 |
publicationDate |
2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-111504758-A |
titleOfInvention |
Method for inactivating and extracting acid-fast bacteria for characterization and/or identification using mass spectrometry |
abstract |
The present invention relates to a method involving the inactivation and/or extraction of acid-fast bacteria (eg, mycobacteria or nocardia) comprising the sequential steps of: (a) obtaining a method known to contain or A test sample that may contain acid-fast bacteria and suspend the test sample in a container containing ethanol and beads; (b) bead milling and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria in the container cells; and (c) subsequently incubating the suspension at room temperature for at least about 3 minutes to inactivate any acid-fast bacteria contained in the test sample. According to the present invention, the test sample can then be pelleted by centrifugation, resuspended with formic acid, added with acetonitrile, and subjected to mass spectrometry for the characterization and/or identification of acid-fast bacteria. |
priorityDate |
2012-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |