http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111504758-A

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publicationDate 2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111504758-A
titleOfInvention Method for inactivating and extracting acid-fast bacteria for characterization and/or identification using mass spectrometry
abstract The present invention relates to a method involving the inactivation and/or extraction of acid-fast bacteria (eg, mycobacteria or nocardia) comprising the sequential steps of: (a) obtaining a method known to contain or A test sample that may contain acid-fast bacteria and suspend the test sample in a container containing ethanol and beads; (b) bead milling and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria in the container cells; and (c) subsequently incubating the suspension at room temperature for at least about 3 minutes to inactivate any acid-fast bacteria contained in the test sample. According to the present invention, the test sample can then be pelleted by centrifugation, resuspended with formic acid, added with acetonitrile, and subjected to mass spectrometry for the characterization and/or identification of acid-fast bacteria.
priorityDate 2012-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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