| abstract |
The invention belongs to the technical field of molecular biology, and relates to four kinds of Brucella (Brucella sheep, Brucella bovis, Brucella suis and Brucella canis) that can infect dogs. Re-PCR detection primers, kits and methods. Only one pair of primers can amplify 352 bp and 709 bp respectively for Brucella rough (B. canis lacks 357bp) and Brucella smooth (bovine, sheep and pig) Effective identification; and the primers can effectively avoid the generation of primer dimers and hairpin structures, ensure efficient PCR amplification, and enhance the specificity and sensitivity of the detection technology. |