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publicationDate 2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111500731-A
titleOfInvention A kit for early diagnosis, detection or screening of thyroid cancer and its using method and application
abstract The invention discloses a kit for early diagnosis, detection or screening of thyroid cancer, a method of use and application thereof. The kit includes specific primers and probes for detecting or measuring the methylation status or level of one or more specific genes in the DNA of the test sample, the specific genes being BNC1 and ACTB. The kit of the invention can significantly improve the positive detection rate and specificity (true negative rate) of early-stage thyroid cancer, and can improve the detection sensitivity of biomarkers to picogram/nanogram DNA molecules. The improvement of detection sensitivity is achieved by optimizing Specific nucleotide sequence primers and DNA probes and improved plasma-free DNA bisulfite treatment method to achieve.
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