http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111500682-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db9efde660b78f98b102046e09d07ab7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-658 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
| filingDate | 2020-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b865c2c264886ed3b3284788586b32e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2904311527a101392b54efc2537c79a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37b538cec259a106187dab9504ab24a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f20dc3eb35a1ba4b57b28a132ccc4086 |
| publicationDate | 2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111500682-A |
| titleOfInvention | DNA hybridization detection method based on surface-enhanced Raman scattering spectroscopy |
| abstract | The invention discloses a DNA hybridization detection method based on surface-enhanced Raman scattering spectroscopy, comprising the following steps: (1) preparing a 30-nanometer-diameter silver nanoparticle suspension; (2) preparing a thiolated DNA probe add to the suspension prepared in step (1); (3) add the DNA sample to be tested into the suspension of silver nanoparticles with the DNA probe fixed in step (2); (4) to step ( Add magnesium sulfate solution to the suspension obtained in 3), and the negatively charged silver nanoparticles and DNA probes will cluster under the bridging action of divalent magnesium ions to form a sandwich cluster structure. The cluster-structured centrifuge tubes were fixed to the glass tube supports for Raman spectroscopy measurements. The invention not only ensures the non-destructive detection of the DNA test product in the DNA measurement process, but also realizes the reliable distinction of the DNA hybrid signal, and has high measurement accuracy, high speed and low cost. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109929748-A |
| priorityDate | 2020-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.