| abstract |
The invention relates to the technical field of extracting and separating biological macromolecules from vegetable oil, in particular to a method for simultaneously extracting oil, protein and polysaccharide in rice bran by three-phase extraction technology. The present invention includes adding solid salt and tert-butanol and fully mixing with rice bran slurry under the combined action of salting out, plasma precipitation, co-solvent precipitation, etc. to form three phases, the upper phase is evaporated under reduced pressure to obtain grease, and the middle phase is dissolved The purified protein is obtained after dialysis and drying in the buffer solution, and the purified polysaccharide is obtained after the lower phase is dialyzed and dried. The invention provides a simple, efficient, economical and green three-phase extraction technology, realizes the simultaneous extraction of oil, protein and polysaccharide in rice bran, and has high product yield and good quality. The invention can directly act on the rice bran slurry, can be operated at room temperature, and has the advantages of high separation efficiency for target products, wide application range, easy expansion and the like. |