http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111499765-B
Outgoing Links
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filingDate | 2020-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-111499765-B |
titleOfInvention | Coronavirus fusion protein and preparation method and application thereof |
abstract | The invention belongs to the technical field of antigen preparation processes, and particularly relates to a coronavirus fusion protein, and a preparation method and application thereof. The coronavirus fusion protein provided by the invention is constructed into an insect cell expression vector after the 2019-nCoV S protein RBD structure domain nucleotide sequence and the 2019-nCoV N protein nucleotide sequence are synthesized in a whole gene manner, a recombinant plasmid containing the 2019-nCoV N protein and the 2019-nCoV S protein RBD structure domain sequence is prepared, a recombinant bacmid containing the fusion protein gene sequence is obtained after the recombinant plasmid is transformed into escherichia coli, and the recombinant bacmid transfects host cells, so that the fusion expression of the 2019-nCoV N protein and the 2019-nCoV S protein RBD structure domain is completed. The expression process of the fusion protein is simple, and when the fusion protein is used in a 2019-nCoV antibody detection kit, the sensitivity of the detection kit is remarkably improved, the specificity is also improved, the detectable rate is efficiently improved, and the overall use performance is more optimized. |
priorityDate | 2020-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 486.