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publicationDate 2020-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111494651-A
titleOfInvention A kind of nematode larvae viability detection method and application
abstract The invention relates to the field of larval viability detection, and specifically discloses a nematode larvae viability detection method and application. The method for detecting the viability of nematode larvae of the present invention includes: (1) dyeing the nematode larvae with methylene blue as a dye; (2) after 5-7 minutes, determining the viability of the nematode larvae according to the color of the nematode larvae. When the nematode larvae are blue or light blue, the nematode larvae are determined to be dead or low-viability larvae; when the nematode larvae are unstained, the nematode larvae are determined to be high-viability larvae. The invention uses MB as a dye to visually identify the vitality of nematode larvae in the digestive tract according to the effect of dyeing depth, is convenient, fast, and low-cost, can effectively avoid differences in subjective judgment, and provides a method for studying the efficacy and drug resistance of anthelmintics. It is of great significance for the selection of sensitive drugs to guide clinical medication.
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