http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111487227-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6fb71722df102c14da56d32f58d79bf |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2020-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0149f7aec6299b194a164f48741802cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c2c7c8d815d469480a23f1e9fdeb68e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85ffa4d8bfc0473601b8c2021adbd7e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf8a5621db7743214e35ec5deee5af70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e554e11655ab3d6d9bf9ec837a400ddd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b98d1708038cf6462b014cd2be05cb9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cccab656b7c91e98bcaed13a8d7017c9 |
| publicationDate | 2020-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111487227-A |
| titleOfInvention | A Fluorescence-Enhanced Sensor for Detecting Pb2+ Concentration in Human Serum |
| abstract | The invention belongs to the technical field of biosensors, and relates to a fluorescence-enhanced sensor for detecting the concentration of Pb 2+ in human serum and a fluorescence analysis method thereof. The method is based on parallel G-quadruplex-induced 4'-aminomethyl-4,5',8-trimethylpsoralen (AMT) fluorescence quenching, and lead ions compete with psoralen for aptamer binding , resulting in the conversion of AMT‑aptamer parallel G‑quadruplex to Pb 2+ ‑aptamer antiparallel G‑quadruplex to release psoralen, constructing a fluorescence-enhanced sensor. Under optimal conditions, the developed aptamer sensor exhibits high selectivity and good accuracy in the range of Pb 2+ concentration from 10 nM to 300 nM, and the limit of detection (LOD) is calculated to be 1.98 nM by the 3σ/K formula. The method is applied to the analysis of human serum for the detection of Pb 2+ by standard addition, which has high sensitivity and selectivity, and has practical application value. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114703256-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113109305-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114703256-A |
| priorityDate | 2020-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.