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filingDate 2019-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-111484961-A
titleOfInvention A genetically engineered bacterium producing 5α-androstanedione and its application
abstract The invention belongs to the technical field of biocatalysis, and in particular relates to the construction and application of genetically engineered bacteria directly transformed from cheap substrate phytosterol (PS) to 5α-androsanedione (5α-AD) in one step. In the present invention, the 5α-reductase gene from Treponema pallidum is heterologously expressed into the mycobacteria that mainly produce androstenedione (AD), so as to realize one-step biotransformation from phytosterol to 5α-AD, thereby realizing 5α-AD green and efficient production.
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