| abstract |
The invention discloses an improved determination method for nitrite reductase in soil. In the presence of a hydrogen donor and anaerobic conditions, the reaction of nitrite and p-sulfanilic acid-α-naphthylamine reagent is used to measure The change of NO 2 ‑ ‑N before and after the enzymatic reaction to represent the nitrite reductase activity. Using a vacuum dryer instead of a decompressed conical flask as a culture vessel can not only ensure that the culture environment is anaerobic, but also simplify the operation process and reduce data errors caused by differences in culture conditions. At the same time, adding a certain amount of KClO 3 in the test and standard curve preparation can inhibit the oxidation of NO 2 ‑ to NO 3 ‑ , reduce the loss of NO 2 ‑ , and improve the data accuracy. The method of the invention has high reproducibility, is simple and easy to implement, reduces errors, makes the measurement results between parallel processing more stable, and the measurement results are closer to the real value. |