| abstract |
The invention provides a genetic risk gene detection method for female fertility based on a risk assessment model, which includes: determining target genes and sites for gene detection; preparing specific multiplex PCR primers or gene chips; Detect DNA data in combination with gene chip detection or the combination of multiplex PCR amplification and high-throughput sequencing; perform bioinformatics analysis on DNA data to obtain the genotype of the target site; use compound random forest algorithm to build a risk assessment model And perform calculations to obtain risk scores for different genotypes, phenotypes and diseases, so as to obtain test and evaluation reports, genetic counseling and health guidance for patients. Non-specific amplification such as pseudogenes is excluded, and the specificity of the test results is high; all genes and loci have been strictly verified and authoritative, which reduces the cost of detection and evaluation, facilitates the inspection of the subjects, saves medical resources, and reduces social costs . |