http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111471735-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9add8f0d3e428b7654b0541d170c3e08 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| filingDate | 2020-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_518483bf475a78700d268892267817d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69b9cb10314a4a2fd4b90d088e6c29ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79925c366d0758229a0073428a826c94 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a7a0ec23ef84ce3cdb7fc40e03471d00 |
| publicationDate | 2020-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111471735-A |
| titleOfInvention | A kind of preparation, separation, purification and identification method of pumpkin seed antioxidant peptide |
| abstract | The invention discloses a method for preparing, separating, purifying and identifying a pumpkin seed antioxidant peptide, which is characterized by adding pumpkin seed powder into water, adding an enzyme for enzymatic hydrolysis, removing the oil layer after inactivating the enzyme, and obtaining the pumpkin seed meal; Using the obtained pumpkin seed meal as a substrate, adding enzymes to carry out enzymatic hydrolysis, and the supernatant after the centrifugation of the enzymatic hydrolysis product is freeze-dried to obtain a pumpkin seed antioxidant peptide mixture; the pumpkin seed antioxidant peptide mixture is dissolved in deionized water to reconstitute After that, the pressure at the outlet of the ultrafiltration membrane plate was controlled through membrane packages with different relative molecular weights, and the components collected after ultrafiltration were named according to different relative molecular weights to determine the components with the strongest antioxidant capacity; Dissolve in ultrapure water, and then further separate the antioxidant peptides of pumpkin seeds by gel chromatography column, and use the hydroxyl radical scavenging rate as an indicator to purify and enrich the functional peptides in pumpkin seeds polypeptide step by step; Identification of antioxidant peptides from pumpkin seeds. |
| priorityDate | 2020-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 93.