| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e9297c8f247b3724a04ec3c3130f182 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1029
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-01009 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-525 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-02 |
| filingDate |
2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88513116e633c374a08b8249b9b59afa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d458b9430061dbb38032e63a01c5a91
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d44e1101278219c6bb5595ce1fb03d2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef830fcb2d561de7bfb4bd5b42b6c6e7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0de97090a94c28d3667d7cb8811f545
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9593c695465b51383eaf910f72c96fba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9650d70dbf12e10723f0242286c0d18d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_acdf19f5608bb0bb419aaaf18fa87fe4 |
| publicationDate |
2020-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-111471633-A |
| titleOfInvention |
A kind of genetic engineering high-yielding strain amylase Streptomyces chromogenes and method for improving ε-polylysine production |
| abstract |
The present invention relates to a genetically engineered strain of high-yield ε-polylysine amylase Streptomyces diastatochromogenes CATF (Streptomyces diastatochromogenes CATF) and a method for improving the fermentation level of ε-polylysine, and the construction steps of the high-yield genetically engineered strain As follows: Step 1, construct a plasmid expressing the acetyl-CoA acetyltransferase CATF gene, and the CATF sequence fragment of the gene is controlled by the erythromycin promoter erm * on the pIMEP plasmid; Step 2, obtaining a strain expressing the catF gene, that is, a high-yielding strain Genetically engineered strain of ε-polylysine amylase Streptomyces chromogenes CATF. Experiments have confirmed that the Streptomyces genetically engineered strain has significantly improved the ability of producing ε-polylysine by 27.91% compared with the original strain Streptomyces chromogenes TUST under the same conditions, which provides excellent performance for the production of ε-polylysine. Bacteria. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113897301-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113549587-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113549587-B |
| priorityDate |
2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |