| abstract |
The invention discloses an optimized engineering strain of Saccharomyces cerevisiae with high production of 2'-fucosyllactose. Through metabolic engineering transformation, Saccharomyces cerevisiae can absorb lactose and use GDP-mannose as a precursor to synthesize GDP-rock in the cell Fucose, and through heterologous expression of α-1,2-fucosyltransferase derived from Bacillus cereus, the obtained recombinant strain can produce high 2'-fucosyllactose, and then through subsequent overexpression The enzymes related to GDP-mannose synthesis increased the yield of 2'-fucosyllactose to about 3.8 g/L, which provided an important reference for the industrial production of 2'-fucosyllactose by Saccharomyces cerevisiae. |