http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111455102-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a33f1acd9dac269a59b0cbdf8fad5e6 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2020-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a346c424da8e6fef82ec7ac946f2ad41 |
| publicationDate | 2020-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111455102-A |
| titleOfInvention | Preparation method of capture probe for targeted sequencing of novel coronavirus SARS-CoV-2 genome |
| abstract | The present invention relates to genome-targeted sequencing. A method for preparing capture probes for targeted sequencing of the novel coronavirus SARS-CoV-2 genome, including downloading the SARS-CoV-2 virus reference genome sequence NC_045512.2 from the NCBI database, adopting a design strategy of shingled coverage, according to Parameters such as GC content, Tm value and homology were used to screen probes with a length of 75-120nt, quantitatively evaluate the probe library, and filter out unqualified probe sequences, leaving 249 probes covering the entire genome of the new coronavirus ; Prepare the DNA template of 249 probe libraries, PCR amplification, purify the PCR product; Carry out enzyme digestion and T4 DNA polymerase end smoothing to the purified PCR product, then use Lambda exonuclease to digest double-stranded DNA One of the single strands with 5'-terminal phosphorylation is obtained to obtain biotin-labeled single-stranded DNA; the magnetic bead enrichment method is used to purify the biotin-labeled single-stranded DNA to obtain a modified probe library. The invention reduces the amount of sequencing data, simplifies the data analysis process, and improves the sensitivity and accuracy of the NGS method for detecting the new coronavirus. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112322788-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112195281-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115346606-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112322788-B |
| priorityDate | 2020-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.