| abstract |
The invention provides a primer, a probe and a detection kit for detecting influenza virus H5N1 based on an RAA fluorescence method, wherein the primer and the probe are suitable for RAA fluorescence method detection, the influenza virus H5N1 can be accurately detected, the specificity can reach 100%, the detection kit can conveniently, quickly and accurately identify the influenza virus H5N1, the operation is convenient, the detection time is short, the detection can be completed within 15min, the DNA is not required to be uncoiled through high-temperature denaturation at 95 ℃ like PCR (polymerase chain reaction), then annealing is carried out at 50-60 ℃, finally, the extension is completed at 72 ℃, the detection can be completed only through isothermal amplification at 30-42 ℃, 4-6 primers are not required to be used for amplification at 65 ℃ like AMP L technology, and false positive is easily generated. |