| abstract |
The invention belongs to the technical field of biological detection, and discloses a primer for detecting Alternaria in jet fuel, an application and a detection method. The primers include: outer primers F3 and B3, inner primers FIP and BIP, two loop primers LB and LF; the sequence of the outer primer F3 is shown in SEQ ID NO.1, and the sequence of the outer primer B3 is shown in SEQ ID NO. 2, the inner primer FIP sequence is shown in SEQ ID NO.3, the inner primer BIP sequence is shown in SEQ ID NO.4, and the loop primer LB sequence is shown in SEQ ID NO.5 As shown, the loop primer LF sequence is shown in SEQ ID NO.6. The method of the present invention has the advantages of high sensitivity and strong specificity. |