http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111454171-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2430-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-569 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C251-60 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C251-60 |
| filingDate | 2020-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111454171-B |
| titleOfInvention | Paraquat hapten PH-A, artificial antigen and antibody as well as preparation methods and applications thereof |
| abstract | The invention discloses a paraquat hapten PH-A, an artificial antigen, an antibody, and a preparation method and application thereof. The invention firstly provides a paraquat hapten PH-A, wherein the structural formula of the hapten PH-A is shown as a formula (I): the hapten PH-A has high overlapping degree with the framework structure of the substance to be detected paraquat, thereby effectively improving the immunogenicity of the hapten PH-A; further using an artificial antigen obtained by coupling the hapten PH-A and lactoferrin as an immunogen to immunize alpaca, and screening to obtain a nano antibody Nb2-23 capable of specifically recognizing paraquat; the method for detecting paraquat is established by utilizing the nano antibody Nb2-23, has the detection limit of 0.54 ng/mL-3.86 ng/mL, the semi-inhibitory concentration (IC50) of 1.44ng/mL and the lowest detection Limit (LOD) of 0.34ng/mL, has the advantages of high detection sensitivity, strong specificity, simple operation and short time consumption, and has good application prospect and wide development space in the rapid and effective detection of paraquat. |
| priorityDate | 2020-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 170.