http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111443118-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3278 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 |
| filingDate | 2020-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111443118-B |
| titleOfInvention | A ratiometric electrochemical sensing platform for vibrio detection, its preparation method and application |
| abstract | The invention provides a ratiometric electrochemical sensing platform for Vibrio detection, comprising a working electrode and a modification layer arranged on the surface of the working electrode, the modification layer comprising a resin- The metal chelate layer, the gold nanoparticle layer deposited on the surface of the resin-metal chelate layer and the probe DNA fixed on the surface of the gold nanoparticle layer, the probe DNA is the specific target of Vibrio The complementary sequence of the DNA sequence, the 3' end and the 5' end of the probe DNA are respectively modified with ferrocene and thiol. The ratiometric electrochemical sensing platform provided by the invention has simple operation, quick response, is not affected by sample color and turbidity, and has high detection sensitivity, specificity and reproducibility. This makes the ratiometric electrochemical sensing platform prepared by the present invention show unique advantages that are different from traditional microbiological detection methods, modern real-time fluorescent quantitative PCR detection methods and general electrochemical sensing platforms, and has broad practical application prospects. |
| priorityDate | 2020-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.